It's on frame 2. Unless a very long leader has been left on the film, I doubt it. If the starting frames are on the exterior of the reel, the problem would probably have been on a much higher frame number (large circumference). If the film on the other hand is rolled starting with the start of the frame, it most likely would be protected by the spindle at this point. If it was film touching during processing, the splotch would be uneven...